Comparison between serological and molecular typing for HLA Class II antigens (HLA-DR and DQ)

Abstract

A total of 17 subjects were typed for HLA-DR Class II antigens using one serologic and two DNA typing kits. Our results showed that DNA typing is superior to serologic typing in assigning the correct HLA type. A total of 8 incorrect assignments and 6 missing assignments were noted. Seven HLA-DQ alleles and 1 HLA-DR8 allele were incorrectly assigned. Discrepancies due to a failure in identifying DR8 (2 cases), DQ6 (2 cases) DR 52 (1 case) and DR 51 (1 case) were also observed.